Septic Test Results

Lake and Pond management
Septic Treatment Solution

Septic Treatment System for Home and Commercial Use

Experimental Summary

A comprehensive, test vs. control laboratory study was conducted to confirm the superior results attainable in standard residential septic tanks using Bio Drain and Septic Cleaner (Regular Strength, liquid formulation). This rigorous test was performed for 90 consecutive days in a laboratory setting using Bio Drain and Septic Cleaner (Regular Strength, liquid formulation). This rigorous test was performed for 90 consecutive days treated (TEST) and untreated (CONTROL) septic systems. The results were excellent:

  •  Discharge quality was at least 28% improved in Total Suspended Solids (TSS) with Bio Drain and Septic Cleaner.
  •  Discharge quality was at least 18% improved in Chemical Oxygen Demand (COD) with Bio Drain and Septic Cleaner.
  •  Discharge quality was at least 12% improved in Oil and Grease  (a  measure of fat content) with Bio Drain and Septic Cleaner.

These improvements, though large and exciting in themselves, do not tell the full story. These numbers were calculated from the beginning through the end of a 90-day laboratory study. However, the results as taken over the last 30 days of the study were even more impressive in Bio Drain and Septic Cleaner’s favor.

However you look at it, these impressive improvements in pollution control mean  dramatically cleaner discharge from your septic tank. And with this improved septic tank efficiency, your septic system will run more efficiently for many years. And that is good for both the environment AND your bank account!


This study was performed to demonstrate the superior efficacy of Bio Drain and Septic Cleaner in the septic tank environment. A key consideration was to design the laboratory system with the capacity to simulate some of the expected variations in load that are typical with real septic tanks.

After all, household septic tanks regularly receive large variances in flow, types of chemicals, pharmaceuticals, etc. To account for this, the experimental systems were designed for both continuous and intermittent effluent flow. To ensure that the study met standard scientific procedures, two parallel, identical systems were set up. This way, one would act as a test (treated with Bio Drain and Septic Cleaner), while the other would act as a control (without product added).septic test

Schematic of Septic Tank Pilot Plant

The dimensions of each of the main tanks (Stage 1 and Stage 2) was 38″x11″x11″, with a total volume of about 20 gallons. Two stages were used to act as a baffle (as you would have in a real septic tank) despite the small volumes involved. The intent was that Stage 1 would accumulate most of the solids, grease, and scum, while Stage 2 would be relatively free of solids accumulations.

As stated above, one system was used as a test tank while the other was used as a control. A common holding tank was used for mixing and dispensing synthetic waste water to the laboratory tanks. Treated waste water from each laboratory system flowed to a refrigerated holding tank so that 24-hour composite samples could be taken.

After a thorough literature search, we decided to make a synthetic waste water that would be representative of waste water that typically enters a residential septic tank. For the duration of this experiment, the synthetic waste water was used for the study. The formulation is given below:
The above nutrients were mixed into 50 gallons of tap water. The influent waste water had the following average composition:

Average Enfluent Waste water Composition

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Equipment Operation

Waste water was fed to the laboratory systems two ways: continuous and batch (intermittent). A peristaltic pump was used to continuously add 6 gallons of synthetic waste water per day to each system. Batch addition was performed by adding 3 gallons per day, twice per day, to Stage 1 of each laboratory system. With a total batch addition of 6 gallons per system per day (3 gallons twice per day), and with 6 gallons per day being fed to each system continuously, the total hydraulic load on each system was 12 gallons per day of synthetic waste water. The actual working volume of Stage 1 plus Stage 2 was 36 gallons, so that the hydraulic residence time was 3 days. This is very important, as we wanted to come reasonably close to the expected holding time of a residential septic tank. A residential tank with a volume of 500 to 1000 gallons may have anything from 1 to 5 days of residence time (depending on the number of people living at the residence and the rate of water use). Therefore, the experimental set-up did agood job of mimicking a real septic tank. The peristaltic pump used for continuous feed consisted of a single drive with two discharge lines. The pump is designed as a dual-feed, identical flow rate system. With this equipment, the test and control septic tanks received identical waste water flow each day.

Experimental Procedures

Start Up:

To start the system up, each laboratory train was filled with 34 gallons of synthetic waste water (split between Stage 1 and 2). Two gallons of anaerobic digester sludge from a local waste water treatment plant (WWTP) was added to the 34 gallons of synthetic waste water. The mixture was undisturbed for three days to allow for initial degradation and acclimation. The purpose of the
anaerobic digester material from the WWTP was to establish a functioning biomass that would be found in a well-operating septic system. As septic tanks are mostly anaerobic systems (without oxygen), the sludge from a functioning anaerobic digester is ideal to start up a septic tank with full biochemical function.


After three days of acclimation, effluent feed was initiated. The peristaltic pumping system was used to add continuously at the rate of 6 gallons per day. Batch addition of 3 gallons per system, twice per day, was also started. The batch additions took place at around 8:00 A.M. and 5:00 P.M. each day. The total addition per day per system was therefore 12 gallons, giving a hydraulic resident time of 3 days.

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Test Procedures:

Effluent from each system flowed to a refrigerated holding tank, so that 24-hour composite samples could be conveniently taken. At the end of each 24-hour period, the holding tank contents were blended to give uniform samples for testing. System influent and effluent were tested Monday through Friday for each of: Total Suspended Solids (TSS), Chemical Oxygen Demand (COD), and oil and Grease. Triplicate tests were performed according to standard methods for each of the parameters.

Bio Drain and Septic Cleaner Addition

Septic Medic was added using standard Septic Medic procedures. Regular Strength Bio Drain and Septic Cleaner should be dosed at 16 ounces for a standard residential septic tank (500 gallons). Therefore, with a tank volume of 36 gallons, the dose rate was 1.2 ounces of Bio Drain and Septic Cleaner 35 cc), performed once
every 7 days, starting with day 1 of operation. As with the regular Bio Drain and Septic Cleaner dose (straight to the commode or toilet), the product was added directly to Stage 1 of the Test experimental set-up.


The chart below is a summary of effluent suspended solids vs. time for the test and control laboratory systems. The chart shows continuously improving performance on the Bio Drain and Septic Cleaner treated side:

Effluent Suspended Solids vs Time

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The next chart is a summary of effluent Total COD vs. time. As with effluent SS performance, the two sides (test and control) exhibited similar performance during the beginning of the run (the first 4 weeks). After that, it is 6 clear that the treated side continued to perform better and better compared to the untreated side.

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Effluent Oil and Grease results are shown in the next chart. As with the other two charts above, the Bio Drain and Septic Cleaner results continued to improve compared to the control results as the test proceeded. This is particularly important in considering the likely effect of Bio Drain and Septic Cleaner on long term drain field operation and efficiency.

septic test6


From the various charts, it is clear that the Bio Drain and Septic Cleaner treated system
had significantly better effluent performance than did the control system. The
table below is a summary of the key parameters:

Average Effluent Performance

Test         Control
Total Suspended Solids                                     60               83
Chemical Oxygen Demand (COD)                      262             318
Oil and Grease (Fats,etc.)                                  53               47

These data prove that use of Bio Drain and Septic has a beneficial effect in the septic tank environment. The reduced pollutant content of the effluent will have several advantages, including reduced load on the drain field, reduced odors, less puddling. The bottom line is that with Bio Drain and Septic Cleaner use, your septic tank will discharge cleaner water to the environment than it would otherwise.

Further, it is clear in each of the three charts that the relative performance of the Bio Drain and Septic Cleaner tank improved compared to the control tank as the experiment  continued. The data above show averages for the entire run. However, as the Bio Drain and Septic Cleaner data were clearly improving towards the end of the experiment, the results can be interpreted as the low end of expected improvement. Just as the experiment continued to improve toward the end of the 90-day run, so will your own experience with Bio Drain and Septic Cleaner.

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